A novel, rapid, and sensitive homogeneous sandwich detection method of Glypican-3 as a serum marker for hepatocellular carcinoma.
نویسندگان
چکیده
Based on multiple interactions and fluorescence quenching, we report a novel homogeneous detection method for Glypican-3 which shows a series of significant advantages, including low cost, ease of preparation, rapid response, and high sensitivity and has great potential in the clinical diagnosis of hepatocellular carcinoma and proteoglycan detection.
منابع مشابه
Chemiluminescence enzyme immunoassay based on magnetic nanoparticles for detection of hepatocellular carcinoma marker glypican-3
Glypican-3 (GPC3) is reported as a great promising tumor marker for hepatocellular carcinoma (HCC) diagnosis. Highly sensitive and accurate analysis of serum GPC3 (sGPC3), in combination with or instead of traditional HCC marker alpha-fetoprotein (AFP), is essential for early diagnosis of HCC. Biomaterial-functionalized magnetic particles have been utilized as solid supports with good biologica...
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For detection of hepatocellular carcinoma (HCC) in patients with liver cirrhosis, serum alpha-fetoprotein has been widely used, but its sensitivity has not been satisfactory, especially in small, well-differentiated HCC, and complementary serum marker has been clinically required. Glypican-3 (GPC3), a heparan sulfate proteoglycan anchored to the plasma membrane, is a good candidate marker of HC...
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BACKGROUND AND AIMS The role of glypican-3 (GPC3), a novel serum marker, in differentiating hepatocellular carcinoma (HCC) from non-malignant chronic liver disease and other malignant space-occupying lesions in the liver is largely unknown. The aims of this study were to evaluate its diagnostic role and clinical correlations in patients with HCC. METHODS Six groups were studied which included...
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ورودعنوان ژورنال:
- Chemical communications
دوره 53 90 شماره
صفحات -
تاریخ انتشار 2005